Part:BBa_K1758351:Design

Nickel responsive promoter with RFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We used the codon optimized, synthesized Sequence of RFP under the control of rcnA from iGEM Team BIT 2013( BBa_K549001 ). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of rcnA with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of rcnA and the pSB1C3:

cm_fwd

CGGCATCAGCACCTTGTC

pSB1C3_RFP_rev

CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA

cm_rev

TATACGCAAGGCGACAAG

pSB1C3_rcnA_fwd

TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT

Source

design based on BBa_K549001(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K549001" target="_blank"> BBa_K59001</a>)